| 초록 |
Cell culture is the process of growing cells in controlled conditions. Two-dimensional culture has been the typical paradigm of in vitro cell culture; however, cells have been demonstrated to behave more naturally when cultured in three-dimensional (3D) environments. Cells grow, differentiate, and develop while interacting with very complex 3D structures. Many scaffold-based approaches have recently been developed for 3D cultures. Among various options, cells allow studying the morphological and physiological effects in the target cells, on inducing certain changes. Therefore, cells are used to replace animal testing for toxicological screening of drugs. This lecture is aimed at anyone interested in the application of 3D culture. It offers insights into a wide variety of strategies applying the principles of 3D cell culture using natural polymers for alternative toxicology. 3D cell culture approaches for alternative toxicological model are presented in this lecture |
