| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 가을 (10/09 ~ 10/11, 제주컨벤션센터) |
| 권호 |
44권 2호 |
| 발표분야 |
콜로이드 및 분자조립 부문위원회 I |
| 제목 |
In silico designed fluorescent arbidol for rapid detection of arbidol-resistant influenza virus. |
| 초록 |
Arbidol, which is applied to inhibit viral infection including influenza virus in Russia and China, has been revealed its inhibitory mechanism. Arbidol stabilizes the trimeric structure of hemagglutinin (HA) of non-resistant influenza virus by binding with the hydrophobic pocket sized ~870-1150 Å, while the corresponding pocket in the resistant one is ~90- 150 Å. Based on this fact, we have developed the fluorescentbased detection system of arbidol-resistant influenza virus. First, we determined that which combination of arbidolfluorescent dye would bind with the pocket in HA from the crystal structure of HA in complex with H3N2 and H7N9 viruses, and we found that Arbidol conjugated with Rhodamine 123 (Arb-Rho123) can be applied for the detection. Then Arb-Rho123 is synthesized by EDC-NHS coupling reaction and exposed to the influenza virus. |
| 저자 |
김진영1, 함승주1, 김현욱2, 강아람2, 송대섭2
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| 소속 |
1연세대, 2고려대 |
| 키워드 |
Arbidol; influenza virus; resistant detection
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| E-Mail |
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