| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 |
12권 2호, p.1773 |
| 발표분야 |
생물화공 |
| 제목 |
Efficient expression of small peptides in a cell-free protein synthesis system |
| 초록 |
Despite their clinical and industrial demands, biological production of small peptides has suffered extremely low productivity. In this report, we describe a method for enhancing the expression level of a peptide in a cell-free protein synthesis system. Briefly, through the fusion of the target genes (β-defensin 2 and human epithermal growth factor) with an appropriately engineered downstream box, the productivity of soluble peptides increased remarkably. Moreover, the ‘open’ nature of cell-free synthesis enabled the in situ removal of the extra amino acid residues from the down stream box. By directly using the PCR-amplified genes as the templates for cell-free protein synthesis, the entire process for the expression of target genes was completed in 7 hours. |
| 저자 |
황미연1, 안진호1, 이경호2, 최차용1, 김동명2
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| 소속 |
1서울대, 2충남대 |
| 키워드 |
small-size peptide expression; overlap PCR method; cell-free protein synthesis system
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| E-Mail |
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| 원문파일 |
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