| 학회 | 한국화학공학회 |
| 학술대회 | 2006년 봄 (04/20 ~ 04/21, 대구 인터불고 호텔) |
| 권호 | 12권 1호, p.402 |
| 발표분야 | 생물화공 |
| 제목 | Co-expression of recombinant proteins in cell-free protein synthesis reactions |
| 초록 | For two plasmid-mediated co-expression in E.coli, mutual compatibility of the replication origins should be carefully considered. In addition, it is also required that each plasmid carries the gene encoding different antibiotic resistance. Since cell-free protein synthesis does not rely on the growth of cells, we expected that different plasmid could be expressed in a same reaction mixture without such constraints. However, on the contrary to our expectation, the E.coli-based cell-free protein synthesis system showed "biased" expression of proteins. For example, when two plasmids encoding CAT and GFP were incubated in the same reaction mixture, GFP showed dominant expression while the expression of CAT was substantially repressed. The biased expression was overcome by sequentially expressing the target proteins in a dialysis reactor. We are currently investigating the molecular mechanism by which the different templates compete during cell-free protein synthesis. |
| 저자 | 금정원1, 김태완1, 오린석1, 김동명2, 이경호2, 안진호1 |
| 소속 | 1서울대, 2충남대 |
| 키워드 | cell-free protein synthesis; dialysis; coexpression |
| 원문파일 | 초록 보기 |
