| 초록 |
Herein, thiolated gelatin/PEGDA IPN composite hydrogels were developed for guided skull bone regeneration, and coacervates (Coa) and gelatin microparticles (GMPs) were used as BMP-2 carriers. Both coacervate and GMPs groups showed significantly enhanced osteogenesis of hMSCs. Sustained release kinetics of BMP-2 from IPN composite hydrogels could be controlled by different formulations. For in-vivo bone regeneration, various IPN gel formulations (i.e., control, hydrogel, hydrogel with BMP-2, hydrogel with BMP-2-loaded Coa, and hydrogel with BMP-2-loaded GMPs) were bilaterally implanted into rat calvarial defects. After 4 weeks, rats, treated with Coa or GMP group, showed a significant increase in bone formation. Taken together, Coa and GMPs were effective carriers to maintain the bioactivity of cargo BMP-2 during its sustained release. Consequently, our IPN composite hydrogel system that combines such BMP-2 carriers could effectively promote skull bone regeneration. |
