| 초록 |
Artificial ECM designing is very important in tissue engineering. A porous scaffold mimicking the properties of extracellular matrices (ECMs) was prepared using silk fibroin / alginic acid for bone tissue regeneration. In this study, silk fibroin / alginic acid hydrogel was loaded into a biphasic calcium phosphate (BCP) ceramic, and the resulting scaffold, with unique micro- and macro-porous orientation, was evaluated for bone regeneration applications. After fabrication of the biomimetic scaffold, porosity and compressive strength was measured, that is highly recommended for cancellous bone substitution. Other physical characterizations were carried out by scanning electron microscopy (SEM), EDS, FT-IR, pore size distribution, swelling and degradation behavior. In vitro cyto-compatibility studies were observed by pre-osteoblasts (MC3T3-E1) cell line and cellular viability, morphology and protein expression were checked. The analysis of induction of osteogenic differentiation and the extent of mineralization were also performed using quantitative ALP and calcium assays, respectively. The in vivo characterization was also carried out by immunohistochemistry staining of osteopontin, osteocalcin, and collagen type I. Our study demonstrated that this novel bone substitute is a promising system for bone regeneration. |
