| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.267 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Characterization of Functional META-Pluronic-RGD Exhibiting Temperature-Sensitive Sol-Gel Transition and Cell Culture into Hydrogel |
| 초록 | Pluronics are nontoxic poly(ethylene oxide)-(polypropylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymers which form thermoreversible gels at high concentrations. These gels have received increasing attention over many years as drug delivery systems for topical, transdermal, ophthalmic and implantable applications and biomaterials.1,2 In particular, Pluronics F127 has been most widely used as proper block copolymers in pharmaceutical systems. For thermal behaviors in specific temperature, functional Pluronic (F127) derivatives such as 4-methacryloxyethyl trimellitic anhydride (META)-grafted Pluronic have already been synthesized. In this study, we synthesized temperature-sensitive and cell adhesive triblock copolymers in which Arg-Gly-Asp (RGD) peptide ligand is grafted to META-Pluronic derivatives to obtain META-F127-RGD.3 The resulting META-F127-RGD showed very similar thermosensitive behaviors to F127 and META-F127 (Fig. 1). The results of tube tilting method and light scattering were revealed that the particle size and sol-gel transition of the META-F127-RGD were slightly lower than those of F127 and META-F127. In addition, we have tried to verify the interaction between the synthesized copolymers and specific cells using various diluents and compounds to improve the adhesion and proliferation of the cells in the hydrogel state. The adhesion behaviors of some cells in ligands-grafted hydrogels were evaluated by optical microscopy, viability, proliferation, and histological studies. These peptide ligands-grafted hydrogels that display cell adhesive characteristics are expected to be useful for tissue engineering, DDS, and gene therapy. References 1. N. K. Pandit and J. Kisaka, Int. J. Pharm., 145, 129-136 (1996). 2. P. Holmqvist and A. Brodin, Int. J. Pharm., 194, 103-116 (2000). 2. U. Hersel, C. Dahmen, and H. Kessler, Biomaterials, 24, 4385-4415 (2003). |
| 저자 | 차승환1, 안광덕1, 김종만2, 한동근1 |
| 소속 | 1한국과학기술(연), 2한양대 |
| 키워드 | Thermosensitive hydrogel; Functional Pluronic; RGD; Cell culture |
