| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Rapid Amplification of Nucleic Acids by Photothermal Heating and Concentration in Plasmonic Cavity |
| 초록 |
Fast detection through rapid nucleic acid (NA) amplification are continuously required in the recent Pandemic situation. Conventional polymerase chain reaction (PCR) uses thermocycler and takes about an hour to complete reactions. Here, we suggest a plasmonic cavity (PC), a spherical polymeric structure integrated with gold nanoparticles, as a faster and simpler NA amplification platform via light-induced heat generation by photothermal conversion. First, we investigate that the temperature is maintained to form proper conditions for recombinase polymerase amplification (RPA) and the polymerization reaction is accelerated by thermal gradient-driven concentration of reactants using the PC. Second, we observed the high photothermal conversion efficiency and rapid thermal cycle operated by the pulsed light source reduces the NA amplification time during PCR. As a result, the target DNA can be rapidly amplified to detectable levels in the PC under the light illumination conditions. |
| 저자 |
한승연1, 안현지1, 곽태진2, 김미설1, 최인희1, 김동철2, Luke.P Lee3
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| 소속 |
1서울시립대, 2서강대, 3Harvard Medical School |
| 키워드 |
NA amplification; Plasmonic cavity; Photothermal heating
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| E-Mail |
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