| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 봄 (04/10 ~ 04/11, 대전 컨벤션센터) |
| 권호 |
39권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Hyaluronic acid-ceramide (HA-CE) based Liposomes for enhanced Gene delivery to CD44-positive cancer cells |
| 초록 |
Hyaluronic acid-ceramide (HA-CE) modified liposomes were designed using DOTAP/DOPE for targeted delivery of therapeutic genes to the CD44-overexpressing cancer cells. Liposomes were synthesized with different molar ratios of HA-CE and most efficient formulation had been tested for further in vitro experiments. The size and zeta potential of HA-CE based liposomes was characterized by DLS. Then lipoplex prepared at different N/P ratios showed strong DNA binding affinity of liposomes for target gene delivery. Cytotoxicity of Liposome was evaluated by WST assay for different cell lines such as MDA MB 231 and NIH3T3 cells. In case of NIH3T3 cells, liposome showed negligible cytotoxicity. However CD44 positive cells; MDA MB 231 exhibited little cytotoxic effect because of higher targeting for HA-CE based liposome as compared to CD44 negative NIH3T3 cells. Then we have examined the transfection efficiency of liposome using luciferase reporter plasmid DNA. |
| 저자 |
수딥타 말릭, 최준식
|
| 소속 |
충남대 |
| 키워드 |
liposome; CD44; HACE
|
| E-Mail |
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