| 초록 |
Rheumatoid arthritis (RA) is a chronic inflammatory disease causing the inflammation of synovial membrane and the articular cartilage destruction. In this work, hyaluronate - gold nanoparticle / Tocilizumab (HA-AuNP/TCZ) complex was prepared for the treatment of RA. AuNP was used as a drug carrier with anti-angiogenic effect. TCZ is a humanized monoclonal antibody against IL-6R and used as an immunosuppressive drug. HA is known to have cartilage-protective and lubricant effects. HA was modified with cystamine and reduced with DTT to prepare end-group thiolated HA (HA-SH). AuNP was chemically modified with HA-SH and physically modified with TCZ. The formation of HA-AuNP/TCZ complex was corroborated by UV-Vis spectroscopy, dynamic light scattering, and TEM. The therapeutic effect of HA-AuNP/TCZ complex was confirmed in collagen induced arthritis model mice by ELISA, histological, and Western blot analyses. |
