| 초록 |
Early detection of periodontitis and its responsible pathogens has become imperative in oral health. Porphyromonas gingivalis, a leading oral pathogen, is known to render Lys-gingipain (Kgp) enzyme which directly plays role in periodontitis. However, conventional methods such as PCR is costly and time-consuming. Moreover, selective identification of Kgp is yet to be developed. Herein, we designed a simple method to quantitatively detect P.gingivalis by analyzing the acquired fluorogenic intensity of a specific peptide substrate upon cleavage in the presence of Kgp. Its biological activity was verified by comparing the fluorescence intensity of: 1) Lys-Xaa peptide substrate and its isomer, 2) other dental proteinases and 3) saliva samples from healthy and patient subjects. We anticipate that such Lys-Xaa specific proteinase will assist in detecting early stages of periodontitis and ultimately prevent further complications. |
