| 초록 |
We present a vascularized organ platform in which multiple types of human-originated brain cells were cocultured in a 3D microenvironment with perfusable microvasculature. The microvasculature was fabricated by using microneedles as templates. The removal of microneedles after the gelation of the collagen matrix allowed the formation of collagen channels with the identical diameter of microneedles. By seeding the endothelial cells in the luminal surface of the channel and embedding other organ cells in the collagen, a 3D vascularized organ model was prepared. The microvasculature physiology was confirmed through transendothelial permeability, immunofluorescence imaging, and cytokine assay. Diseases such as brain tumor, lung cancer, and COVID-19 were recapitulated in the vascularized organ platform. Pathophysiological features, as well as the efficacy of pharmaceutical candidates were evaluated in the platforms. |
