| 초록 |
In this study, a series of ATP-regenerating energy sources have been compared for their efficiency to support the generation of active proteins in a cell-free synthesis system. We found that the ATP profile and pH during cell-free protein synthesis(CFPS) substantially changes depending upon the methods for ATP regeneration. Among the energy sources examined, creatine phosphate(CP) provided most stable supply of ATP with minimal changes in pH during cell-free protein synthesis reactions. Although the use of CP did not always lead to the synthesis of target proteins at maximal yields, most likely due to the stable maintenance of pH, the proportion of the soluble or active proteins was substantially higher in the reaction mixture utilizing CP. The presented results suggest that the proper choice of energy source is important for the generation of active proteins in the CFPS system. |
