| 학회 | 한국재료학회 |
| 학술대회 | 2020년 가을 (11/18 ~ 11/20, 휘닉스 제주 섭지코지) |
| 권호 | 26권 1호 |
| 발표분야 | B. 나노화학/바이오 재료 분과 |
| 제목 | Extracellular matrix (ECM) integrated βTCP beads for bone regeneration |
| 초록 | Background: Extracellular matrix (ECM) contains proteins and growth factors necessary for cell adhesion, proliferation and intercellular communication. This group of proteins plays a vital role for tissue regeneration by providing homing effect to cells. Decellularized extra cellular matrix bears the same potentials as the least amount of native components and growth factors remain intact. ECM accompanied with biocompatible polymers and bone cement can be used to fabricate more sustainable and biocompatible bone substitute. Method: Allogeneic ECM was prepared from porcine liver tissue by detergent assisted cell removal process followed by pepsin digested ECM hydrogel formulation. The characterization of ECM was performed by biochemical analysis as well as histo-pathological staining. The beads were prepared by extrusion crosslinking procedure. Biocompatibility was assessed by WST based colorimetric analysis and confocal staining at specific time interval. Haptotaxis/cell attachment behavior was evaluated through scanning electron microscope (SEM) and fluorescence imaging. The surface structure and size distribution of different group of beads were demonstrated by SEM. The distribution of every component within the final product was analyzed by Fourier-transform infrared spectroscopy (FTIR) as well as X-ray powder diffraction (XRD). In vitro swelling and biodegradation behaviors were determined by typical PBS uptake and weight loss assay at different time intervals respectively. In vivo bone regeneration by the different group of beads was demonstrated by implantation on rabbit model followed by micro CT analysis and histochemical staining. Results: Complete removal of cell from the liver ECM was ascertained with minimal loss of inherited components e.g. collagen, sGAG, α-elastine etc. The ECM loaded microspheres with a size range 1.15-1.50 mm have significance biocompatibility compared to the control plate. Besides a strong haptotaxis behavior towards the preosteoblast cell line, MC3T3E1, was observed with ECM contained beads. Homogenous distribution of all fabrication materials was confirmed by FTIR and XRD spectra. The group of beads AC, without βTCP and ECM, showed highest in vitro biodegradation behavior while another two groups TAC (with βTCP) and ETAC (with ECM and βTCP) showed moderate degradation behavior. Highest swelling behavior was documented for the TAC and on the contrary, moderate for ETAC and AC. Significant in vivo tissue regeneration capacity was observed with ECM contained bead, ETAC, compared to non ECM contained beads ETAC and AC. Conclusion: ECM based scaffolds were well cited for soft tissue regeneration in literature. But there are more options to explore the potentiality of ECM with hard tissue. Our study developed a novel moldable framework with biopolymers and bone cement that contains significant bone regeneration capacity due to integration of ECM within. |
| 저자 | Shohanur Rahaman1, Kang2, H. J3, Seongsu Park4, Tamanna Sultana5, Jae-Gyoung Gwon6, Byong-Taek Lee3 |
| 소속 | 1Department of Regenerative Medicine, 2College of Medicine, 3Soonchunhyang Univ., 4Cheonan-31151, 5Republic of Korea, 6Institute of Tissue Regeneration |
| 키워드 | ECM; bead; biocompatibility; moldable; haptotaxis; bone regeneration |
