| 초록 |
Considering clinical complications and economic burden due to post-surgical peritoneal adhesions, various thermosensitive injectable strategies have been developed as potential adjuvant for tissue adhesion prevention. In this work, mitomycin C was integrated with tempo oxidized cellulose nanofiber (TOCN) by EDC/NHS-crosslinking followed by combining with methyl cellulose (MC) to develop a thermosensitive hydrogel for anti-adhesion application. TOCN successfully cross-linked with mitomycin C and ensured controlled release of mitomycin from hydrogel throughout 14 days period. Amount of MC (1.5, 2.5, 3.5% w/v) was directly proportional to gelation time and inversely proportional to degradation pattern of hydrogel. The optimized cytocompatible hydrogel (MTmc 2.5) needs only 30±3.56 seconds to render thermoreversible sol-gel (4℃-37℃) phenomenon which exhibits excellent injectability at room temperature. MTmc 2.5 hydrogel ensured complete anti-adhesion efficacy as well as reperitonealization in rat cecal abrasion model compared to normal saline group and rats treated by hydrogel without mitomycin C. Therefore, the developed hydrogel is advancement of state-of-the-art in view of cytocompatibility, mechanical stability, optimum degradation, good injectability, prevention of leakage from surgical sites, and most importantly successful adhesion prevention efficiency. |
