| 초록 |
Two types of glycosyltransferase genes from Stenotrophomonas sp. were cloned by PCR, and then inserted to pET-21b(+) expression vector. The recombinant GT genes were expressed in Escherichia coli and the crude cell extracts were reacted with flavonoid to analyze their abilities for glycosylation. We also analyzed the substrate specificity towards various flavonoids such as apigenin, chrysin, daidzein, genistein, kaempferol, luteolin, naringenin, resveratrol, quercetin and etc. with uridinediphosphate-activated glucose. The formation of flavonoids glucosides were analyzed and confirmed by HPLC. |
