| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 가을 (10/22 ~ 10/24, 대전 DCC) |
| 권호 |
20권 2호, p.1544 |
| 발표분야 |
생물화공 |
| 제목 |
Delvelopment of an amino acid-based 1-propanol production pathway via metabolic engineering in Escherichia coli |
| 초록 |
1-propanol has been considered as a next generation biofuel that can be used as a gasoline substitute. Here we suggest a novel biosynthetic pathway for 1-propanol production using an amino acid overproducing Escherichia coli strain. The highest titer of 1-propanol (3.5 g/L), performed by Atsumi et al., has been produced in metabolically engineered Escherichia coli harboring Methanococcus jannaschii citramalate synthase (CimA), which converts pyruvate directly into 2-ketobutyrate. The previously reported L-threonine overproducing E. coli TH20 strain was used in this study. Deletion of competing pathways and in silico flux response analysis for the carbon source optimization successfully generated an E. coli strain producing 1-propanol. Additional metabolic engineering of the resulting strain further improved the titer. [This work was supported by the Advanced Biomass R&D Center of Korea (NRF-2010-0029799) through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST). Further supports by BioFuelChem, EEWS program of KAIST.]. |
| 저자 |
천승우, 최용준, Jin hwan Park, Tae Yong Kim, 이상엽
|
| 소속 |
KAIST |
| 키워드 |
1-propanol; metabolic engineering; Escherichia coli; Methanococcus jannaschii; citramalate synthase; 2-ketobutyrate; L-threonine
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
