| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | 5-aminolevulininc Acid Production Improvement in Escherichia coli by Elaborate Carbon Flux Redistribution |
| 초록 | Microbialfermentation of 5-aminolevulinic acid (ALA) has received much attention becauseof its potential in clinical applications. Overexpression of the relatedenzymes and an analogue transporter yielded remarkable achievements in ALAproduction. There is still significant room for carbon flux optimization toimprove ALA production. This study aimed for precise carbon flux optimizationfor high ALA production in E.coli expressing the ALAbiosynthetic pathway. Initially, genes hemA and hemL were overexpressed. Then, the tricarboxylic acid (TCA) cycle was knocked-out forre-distribution of carbon flux toward the ALA production, additional precisetuning of carbon flux to the glyoxylate shunt by varying the transcriptionalstrength of aceA led to substantially improved cell growth and ALA production.Thus, this precise tuning of the glyoxylate cycle in a quantitative mannershould also enable efficient production of other valuable products derived fromthe TCA cycle. |
| 저자 | 백선우1, 예대열1, 임현규1, 노명현1, 백동엽1, 박성훈2, 서상우3, 정규열1 |
| 소속 | 1포항공과대, 2UNIST, 3서울대 |
| 키워드 | ALA; 5-aminolevulinic acid; Glyoxylate shunt; Flux distribution |
