| 초록 |
The detection of blood circulating biomarkers would provide several advantages over current invasive techniques for early diagnosis of cancer and monitoring treatment. Circulating tumor cells (CTCs) are shed into the peripheral blood from the solid tumor, and has been studied as biomarkers for diagnostics. Furthermore, the occurrence of epithelial-mesenchymal transition (EMT) of these tumor cells has been hypothesized as key mediators of metastasis, exhibiting mesenchymal (stem cell-like) phenotype and expression profiles. In this study, we developed a nanoparticle hybridization method for detecting CTCs as well as their molecular profiles. We performed asymmetric amplification of cDNA derived from cancer cells cultured in vitro to obtain single-strand DNA (ssDNA) of the specific targets, followed by hybridization with nanoparticle-DNA probes for sensitive and specific detection. |
