| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 |
17권 2호, p.1454 |
| 발표분야 |
생물화공 |
| 제목 |
Trypsin Coatings on Magnetically-Separable Nanomaterials for Protein Digestion |
| 초록 |
In the bottom-up proteomic analysis, proteins are digested before LC-MS analysis is performed for the protein identification. However, the poor stability of protease (such as trypsin) has hampered the efficient and automated process of protein digestion. This presentation will introduce the enzyme coating approach that consists of covalent enzyme attachment and enzyme crosslinking. The resulting enzyme coatings are highly stable with high enzyme loadings. The trypsin coatings on nanofibers (1) and magnetic nanoparticles (2) will be introduced as a successful example of enzyme coatings. Their potential application in protein digestion will be discussed with several examples of successful protein digestion results. Facile capture of trypsin coatings on magnetically-separable nanomaterials will enable an easy recycle and potentially development of automated protein digestion process for proteomic analysis. |
| 저자 |
이병수1, 장문석1, 김병찬2, 이상원1, 김중배1
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| 소속 |
1고려대, 2KIST |
| 키워드 |
Enzyme stabilization; Nanobiocatalysis; Protein digestion; Magnetic separation
|
| E-Mail |
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| 원문파일 |
초록 보기 |
