| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.756 |
| 발표분야 |
[주제 5] 친환경 플라스틱 |
| 제목 |
Improving 5-aminolevulinic Acid Production in Escherichia coli by Elaborate Flux Distribution of Glyoxylate cycle |
| 초록 |
Microbial fermentation of 5-aminolevulinic acid (ALA) has received much attention due to its potential in clinical applications. Overexpression of the related enzymes and an analogue transporter yielded remarkable improvement in ALA production, accompanied by deciphering its regulation. Despite this, there is still significant room for carbon flux optimization to improve ALA production. This study was aimed for precise carbon flux optimization for high ALA production in Escherichia coli expressing the ALA biosynthetic pathway. Initially, genes hemA and hemL were overexpressed with strong promoters and synthetic 5'-untranslated regions (5'-UTR). Then, the tricarboxylic acid (TCA) cycle was knocked-out to re-distribute the carbon flux towards the ALA production. Additional precise tuning of carbon flux towards the glyoxylate shunt by varying the transcriptional strength of aceA led to substantially enhanced cell growth and ALA production. Thus, this precise tuning of the glyoxylate cycle in a quantitative manner should also enable the efficient production of other valuable products derived from the TCA cycle. |
| 저자 |
백선우1, 예대열1, 임현규1, 노명현1, 백동엽1, 박성훈2, 서상우3, 정규열1
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| 소속 |
1포항공과대, 2unist, 3서울대 |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
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