| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Efficient 5-aminolevulininc Acid Production by Elaborate Flux Distribution in Glyoxylate Shunt Pathway in Eshcerichia coli |
| 초록 |
Microbial fermentation of 5-aminolevulinic acid (ALA) has received attention due to its potential in clinical applications. Overexpression of the related enzymes and its transporter yielded remarkable achievements in ALA production, accompanying with deciphering its regulation. In spite of this, there is still significant room for carbon flux optimization to improve ALA production. This study aimed for precise carbon flux optimization for high ALA production in Escherichia coli. Initially. genes hemA and hemL were overexpressed with strong promoters and synthetic 5'-untranslated regions. Then, TCA cycle was knocked-out for re-distribution of carbon flux toward the ALA production, additional tuning of carbon flux to the glyoxylate shunt by varying the transcription of aceA led to substantially improved cell growth and ALA production. This tuning of the glyoxylate cycle in a quantitative manner should also enable efficient production of other valuable products derived from the TCA cycle. |
| 저자 |
예대열1, 임현규2, 노명현2, 백동엽1, 박성훈3, 서상우4, 정규열1
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| 소속 |
1포항공과대, 2UCSD, 3울산과학기술원, 4서울대 |
| 키워드 |
Metabolic engineering; 5-Aminolevulinic acid; Glyoxylate shunt; Flux distribution
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| E-Mail |
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