| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Fine tuning the flux in glyoxylate shunt to improve 5-Aminolevulinic acid production in Escherichia coli |
| 초록 |
Microbial fermentation of 5-aminolevulinic acid (ALA) has received much attention because of its potential in clinical applications. Overexpression of the related enzymes and an analogue transporter yielded remarkable achievements in ALA production, accompanying with deciphering its regulation. However, there is still significant room for carbon flux optimization to improve ALA production. This study aimed for precise carbon flux optimization for high ALA production in Escherichia coli. Initially. Genes hemA and hemL were overexpressed with strong promoters and synthetic 5'-untranslated regions (5'-UTR). Then, the tricarboxylic acid (TCA) cycle was knocked-out for re-distribution of carbon flux toward the ALA production, additional precise tuning of carbon flux to the glyoxylate shunt by varying the transcriptional strength of aceA led to substantially improved ALA production. |
| 저자 |
예대열1, 임현규1, 노명현1, 백동엽1, 박성훈2, 서상우3, 정규열1
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| 소속 |
1포항공과대, 2울산과학기술원, 3서울대 |
| 키워드 |
5-Aminolevulinic acid; Glyoxylate shunt; Flux distribution
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| E-Mail |
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