| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Particulate Delivery of Vascular Endothelial Growth Factor Plasmid from Biodegradable Polycaprolactone/Polyethylenimine Electrospun Nanofibers for the treatment of Myocardial Infarction |
| 초록 |
In this study, we are presenting nanofiber mediated gene delivery for myocardial infarction. Branched polyethylenimine cross-linked via disulfide bonds (ssPEI) complexed with VEGF were immobilized on the electrospun polycaprolactone (PCL)/ polyethylenimine (PEI) for local gene exppression. Reporter genes luciferase and therapeutic gene VEGF were tested to evaluate the transfection efficiency of ssPEI nanoparticles immobilized on the nanofiber surface. Our results demonstrated that the delivery of therapeutic genes from biodegradable nanoparticles immobilized on the nanofiber represented minimal cytotoxicity to NIH/3T3 fibroblast cells and H9C2 myoblasts than branched PEI 25kDa did. According to luciferase assay, RT-PCR, this vector showed high transgene expression efficiency to reporter gene and VEGF gene. Surface-mediated delivery of DNA nanoparticles did not adversely affect cell growth and also facilitated the expression of transgene inside the cells. |
| 저자 |
차혜련1, MUTHUNARAYANAN MUTHIAH1, 박인규1, 손세진2, 김원종2, 안영근1, 조종수3, 선우훈3, 정종훈3
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| 소속 |
1전남대, 2포항공대, 3서울대 |
| 키워드 |
Nanofiber; ssPEI; surface-mediated delivery; Vascular endothelial growth factor
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| E-Mail |
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