| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.61 |
| 발표분야 |
고분자 |
| 제목 |
Preparation of monodisperse PEGDA-organoclay composite hydrogel particles for E. coli encapsulation using microfluidic device |
| 초록 |
We propose an effective strategy for E. coli encapsulation in polyethylene glycol diacrylate (PEGDA)-organoclay composite microdroplets using a microfluidic device and self-gelation process. PEGDA is well known polymer for highly porous structure used in biological researches because of its biocompatibility and hydrophilic properties. As the organoclay solution introduced to the PEGDA solution, PEGDA chains are spontaneously crosslinked with each other and formed a hydrogel type polymer matrix. Microfluidics can offer a sophisticated platform to produce the uniform sized spherical PEGDA-organoclay droplets of micron size in a single-step process. This unique process is successfully applied to entrap E. coli inside of PEGDA and organoclay composite matrix. Green fluorescent protein (GFP) is efficiently expressed inside the microdroplet after introduction of iso-propyl-β-D-thio-galactopyranoside (IPTG). Our results have shown that PEGDA /organoclay composite possesse non-toxic, porous and high biocompatible properties. |
| 저자 |
왕계원1, 이경균2, 박태정3, 이영철1, 양지원1, 이석재4, 김도현1
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| 소속 |
1KAIST, 2KAIST / NEMS-Bio Team of NNFC, 3Bioprocess engineering research center of KAIST, 4NEMS-Bio Team of NNFC |
| 키워드 |
Microfluidics; Droplet; Organoclay; Polyethylene glycol diacrylate; E. coli; Hydrogel
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| E-Mail |
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| 원문파일 |
초록 보기 |
