| 학회 | 한국화학공학회 |
| 학술대회 | 2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 | 14권 2호, p.2700 |
| 발표분야 | 생물화공 |
| 제목 | Cell Chip Based on Electrochemical Detection to Analyze the differentiation of Mouse Embryonic Stem Cell |
| 초록 | In this study, we made the chips for the cells using synthetic oligopeptide and nanopattern. The peptide was modified by cysteine for the direct self assembly on the gold chip and we investigated the aspect of cells on the nanopattern to decide the best pattern size for cells. And the voltammetric behaviors of MES cells using 1-Naphthyl Phosphate (NP) showed a good linear relationship with cell number. As an application, electrochemical detection of analyzing differentiation of MES cell was shown. These results indicate that RGD peptide self-assembled layer mediated cell immobilization technique and voltammetric signal analysis system can be applied to construct the stem cell chip for the diagnosis, drug detection, and on-site monitoring. This research was supported by Seoul R&BD Program(10816) and by the Nano/Bio science & Technology Program (M10536090001-05N3609-00110) of the Ministry of Science and Technology (MOST) and by Ministry of Environment of the Republic of Korea as "The Eco-technopia 21 project" |
| 저자 | 예철헌, 김현희, 김정호, 최정우 |
| 소속 | 서강대 |
| 키워드 | cell chip; mouse embryonic stem cell; differentiate; electrochemical detection |
| 원문파일 | 초록 보기 |
