| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.344 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Optical Detection of Small Charged Molecules via Surface-enhanced Raman Spectroscopy for Biological Conversion |
| 초록 |
Real-time monitoring of small charged molecules during biological conversion holds great importance for the optimization of conversion efficiency and reproducibility of the bioprocess. In general, chromatography-based detections such as HPLC and GC are used for the detection of such molecules during the bioconversion. However, these methods are time-consuming, typically taking more than 10 minutes due to the retention time for separation. Furthermore, off-line sampling is necessary for the analysis which has a risk of causing contamination. For these reasons, a novel method for real-time monitoring for bioconversion is required. Here, we propose the optical detection of small charged molecules in biological conversion via surface-enhanced Raman spectroscopy. Such molecules are attracted by the surface ligands of plasmonic nanostructure. As a result, molecules are preconcentated on the surface of the nanostructure, increasing the number of molecules affected by strong field enhancement at the proximity of the surface. The effect of electrostatic attraction is systematically studied thorugh the measurement of zeta-potentials and Raman signals. |
| 저자 |
황금래, 장지한, 장정우, 이영재, 정광영, 김유연, 강태욱
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| 소속 |
서강대 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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