학회 | 한국화학공학회 |
학술대회 | 2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
권호 | 21권 1호, p.438 |
발표분야 | 생물화공 |
제목 | Optimization of media for enhanced ethanol production by Enterobacter aerogenes ATCC 29007 |
초록 | A remarkable increase of biodiesel production has been shown, recently. Also, crude glycerol which is a by-product of the biodiesel was rapidly increased. The previous work indicated that additional casein hydrolysate of media effectively enhanced ethanol production which was proved by expression level of alcohol dehydrogenase (adhE). Three-dimensional(3D) plots for all related factors were also indicated from partial differentiation. Three-dimensional(3D) mesh plots of the results were analyzed using Response Surface Methodology (RSM). The F value and P value were 13.53 and 0.0001, respectively. The coefficient of determination (R2) of the conversion rate model was excellent at 0.926, while the coefficient of variation (CV) was 18.96%. The major factors for RSM with the optimized values were as follows: 8.99 g/L peptone, 5.97 g/L (NH4)SO2, 13.92 g/L casein hydrolysate and 37 g/L glycerol. The conditions of fermentation were performed at initial pH of 6 and 37 oC, 180 rpm for 24 h. Finally, the ethanol concentration was about 0.89 yield under optimized conditions. |
저자 | 이주훈, 이상준, 유하영, 이자현, 최한석, 김동섭, 박찬호, 이수권, 김승욱 |
소속 | 고려대 |
키워드 | Casein Hydrolysate; Ethanol; Response Surface Methodology(RSM) |
원문파일 | 초록 보기 |